Primer

Part:BBa_K4717001:Design

Designed by: Jun Zhou   Group: iGEM23_BIBS-beijing-china   (2023-10-10)


GLUT1 primer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The result by Primer3 using 1-based sequence positions: OLIGO start len tm gc% any 3' rep seq LEFT 12244 20 59.99 55.00 3.00 5.00 12.00 TCCCACTTGGCAGACTCTCT RIGHT 12486 20 60.01 60.00 3.00 8.00 11.00 ACTGGACCCAGTCCTGACAC SEQUENCE SIZE: 33516 INCLUDED REGION SIZE: 33516 PRODUCT SIZE: 243, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00 PRIMER PICKING RESULTS FOR NC_000001.11:c42958868-42925353 Homo sapiens chromosome 1, GRCh38.p14 Primary Assembly


Source

In this project, we used Primer3 (https://bioinfo.ut.ee/primer3-0.4.0/) to design the primer sequence based on information of NCBI nucleoside database.

References